Repeated Sub-anaesthetic dose of Ketamine Elevates Superoxide Dismutase in Pharmacological Model of Schizophrenia-Like Phenotypes in Mice
Objective: Thestudy evaluatedbehavioural phenotypes and the level of Superioxde Dismutase activity in repeated sub-anaesthetic dose of ketamine administered to model schizophreniain animal study. Method:Animals were divided into groups (n=6), control groupreceived distilled water (10mL/kg)as vehicle (VEH), Ketamine treated group (KET) received sub-anaesthetic dose of ketamine (20mg/kg) for 14 days consecutively.Animals in risperidone treatedgroup(KET+RISP) were pre-treated with sub-anaesthetic dose of ketamine(20mg/kg)alonefor 7 consecutive days, and from day 8-14, risperidone (0.5mg/kg) was administered 1 hour post-ketamine treatment. All treatment were administered intraperitoneally(i.p).Twenty-four (24) hours after the last treatment, Behavioural phenotypes(locomotor activity and cognition)were assessed in locomotor activity cage and elevated maze plus. Thereafter level of superioxde dismutase(SOD)activity was evaluated in homogenized brain tissue of each mouse using spectrophotometric analysis. Result: KET groupshowed significant (p<0.05) increase in movement counts and number of rearing eventsin locomotor activity test , also prolonged latency to enter the open armsin cognitive assessment compared to animals that received distilled water (10mg/kg), and risperidone (0.5mg/kg)treatment. The level of Superioxide Dismutase (SOD) activity was significantly elevated in KET group compare with vehicle control and risperidone treatedanimals. Conclusion: Repeated dose of ketamine may pose differential effect onendogenous antioxidant system which may elevate superioxide dismutase activity in ketamine induced schizophrenic rodent as positive control mechanism.