Regenerative potential of human enteric glia in a preclinical model of acute brain injury
Acute brain injuries are characterized by extensive tissue damage, resulting in neuronal loss and severe functional deficits in patients. Self-regeneration is insufficient for the repair of damaged tissue. Therapies based on exogenous cells may offer a promising approach. In this study, we evaluated the feasibility of transplanting exogenous human enteric glia (hEG) in a preclinical model of brain injury. hEG were isolated, expanded and administered intranasally in brain-injured immunocompetent rats. hEG satisfied the safety criteria for cell therapy and were well tolerated. Additionally, hEG migrated to the injured area of the brain, where they enhanced endogenous angiogenesis and neurogenesis, contributing to tissue regeneration. Notably, hEG generated neurons that engrafted and integrated into the brain tissue. These neurons were enveloped by host oligodendrocytes and formed synaptic connections within the host tissue. Our findings provide evidence that hEG have regenerative potential and might be safely used for repair strategies in brain injury.