Gladiolus alucraensis Sağıroğlu & Eker sp. nova (Iridaceae) is described and illustrated from Alucra District of Giresun Province in northern Anatolia, Turkey. It is morphologically close to G. aladagensis and G. kotschayanus, but differs from them by numerous macromorphological flower characters and micromorphological seed characters. The diagnostic morphological features, distribution, habitat, and conservation assessment of the new species are presented. The micromorphological characteristics of the seeds of G. alucraensis, G. aladagensis and G. kotschyanus mericarps were examined using LM and SEM analyses.